Studies of prostaglandin E2 formation in human monocytes
نویسنده
چکیده
.................................................................................................i LIST OF PUBLICATIONS .......................................................................ii ABBREVIATIONS ...................................................................................iii TABLE OF CONTENTS ......................................................................... iv INTRODUCTION..................................................................................... 1 Monocytes and their role in inflammation............................................. 1 Arachidonic acid and eicosanoids.......................................................... 2 From phospholipids to the final product prostaglandin E2 ................... 2 Phospholipase A2 .................................................................................... 3 Intracellular phospholipase A2 ..............................................................................4 Cytosolic phospholipase A2 .............................................................................4 Calcium independent phospholipase A2 .......................................................6 Secretory phospholipase A2 ...................................................................................7 Cyclooxygenases ..................................................................................... 8 Cyclooxygenase-1.....................................................................................................9 Cyclooxygenase-2...................................................................................................10 Cyclooxygenase-3...................................................................................................10 Differences between cyclooxygenase-1 and cyclooxygenase-2 ....................11 Cyclooxygenase inhibitors ...................................................................................12 Prostaglandin E synthases.................................................................... 12 Microsomal prostaglandin E synthase-1 ...........................................................13 Microsomal prostaglandin E synthase-2 ...........................................................14 Cytosolic prostaglandin E synthase ...................................................................14 Prostaglandin E2 ................................................................................... 15 PRESENT INVESTIGATION ............................................................... 17 Background and aim............................................................................. 17 Methodology ......................................................................................... 19 Results and discussion.......................................................................... 22 Conclusions........................................................................................... 31 ACKNOWLEDGEMENTS ..................................................................... 32 REFERENCES ........................................................................................ 33
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